Just to affirm that locality of any breeding population will exert its influence, irrespective of whether or not you have the more heterozygous population scheme of the promiscuously pollinated approach (PPA) or the inbred heirloom approach (IHA). As noted, the former will have, and be likely to maintain, greater genetic diversity than the latter. Yet at the end of the day, a person maintaining a PPA population in, say, Yuma, Arizona will likely have different allele frequencies after several generations than a person does using the same approach and same founder population if they have been doing their project in Winnipeg, Manitoba, even if both have been diligent about samples size during pollination. Also, I remain fascinated by what constitutes (genetically speakding) any given heirloom variety today. If one were to take a small seed sample from all of the independent growers/gardeners around the world of what they call, for example, 'San Marzano' tomato, how genetic diversity would exist *within* each seed lot and how much diversity would exist *between* the different seed lots? Moreover, even if a population is isolated from others of its species and is inbreeding, to what extent is mutation AND acceptance of DNA from other species in the same Genus driving the creation of new alleles, some of which may offer selective advantage in that locality and/or new traits to the population?
Great project, Joseph, and good luck at the conference!