• Post Reply Bookmark Topic Watch Topic
  • New Topic
permaculture forums growies critters building homesteading energy monies kitchen purity ungarbage community wilderness fiber arts art permaculture artisans regional education skip experiences global resources cider press projects digital market permies.com pie forums private forums all forums
this forum made possible by our volunteer staff, including ...
master stewards:
  • John F Dean
  • Carla Burke
  • Nancy Reading
  • r ranson
  • Pearl Sutton
  • Jay Angler
stewards:
  • Liv Smith
  • paul wheaton
  • Nicole Alderman
master gardeners:
  • Christopher Weeks
  • Timothy Norton
gardeners:
  • thomas rubino
  • Matt McSpadden
  • Eric Hanson

Plant-based Sterilization for Mushroom Lab

 
pollinator
Posts: 418
Location: wanderer
179
forest garden fungi foraging bike homestead
  • Likes 3
  • Mark post as helpful
  • send pies
    Number of slices to send:
    Optional 'thank-you' note:
  • Quote
  • Report post to moderator
So I have successfully been cloning mushrooms and growing mycelium for several years now. However, in my lab I use all sorts of sterilizing products that I'm not too proud of. I've been thinking of ways to sterilize my lab space with less - or even better without - toxic gick. So far I've come up with two solutions - neither of which I have tried yet: 1. Steam sterilization 2. Grain alcohol (rectified spirits [Everclear], vodka, gin, etc).
Thoughts? Has anyone here tried toxin-free lab sterilization? If so, what's worked for you?
Thanks in advance.
STEAM_CLEANER_by_Aaron_Yoo_CC_BY-ND_2.0_flickr.jpg
steam cleaner by Aaron Yoo
steam cleaner by Aaron Yoo
 
pollinator
Posts: 241
65
  • Likes 4
  • Mark post as helpful
  • send pies
    Number of slices to send:
    Optional 'thank-you' note:
  • Quote
  • Report post to moderator
 You could make some strong vinegar with a small hand-full of raisins or whatever... takes less than a month to get some strong stuff.
Keep a 1/2 gallon going at all times and it will probably sterilize any surface very fast.
 
pollinator
Posts: 820
Location: South-central Wisconsin
329
  • Mark post as helpful
  • send pies
    Number of slices to send:
    Optional 'thank-you' note:
  • Quote
  • Report post to moderator
Depending on what's being sterilized, you might be able to cook it in an autoclave or pressure-cooker.
 
Ellendra Nauriel
pollinator
Posts: 820
Location: South-central Wisconsin
329
  • Likes 1
  • Mark post as helpful
  • send pies
    Number of slices to send:
    Optional 'thank-you' note:
  • Quote
  • Report post to moderator
Or, I just remembered, there are UV light sterilizing lamps available. I don't know which brand and style would work best for you, but it might be worth investigating.
 
pollinator
Posts: 225
56
duck forest garden chicken cooking building
  • Likes 1
  • Mark post as helpful
  • send pies
    Number of slices to send:
    Optional 'thank-you' note:
  • Quote
  • Report post to moderator

Ellendra Nauriel wrote:Or, I just remembered, there are UV light sterilizing lamps available.



I actually got a brand new one of these, unopened, lying around here somewhere. Humana sent me one a few months ago due to the whole COVID panic.

These things are fairly cheap - about $25 online. I have zero idea how effective they are.

@MikeKenzie I would trade you my unused UV wand in exchange for two new species of edible/culinary (non-psychodelic) mushrooms I can grow on logs.

(I'm already growing portobella, winecap, lion's mane, and yellow oysters. I'd like two new ones)

I don't have any preference to the format you send it in (e.g. syringe, log, pegs, mason jar).
 
Mike Kenzie
pollinator
Posts: 418
Location: wanderer
179
forest garden fungi foraging bike homestead
  • Mark post as helpful
  • send pies
    Number of slices to send:
    Optional 'thank-you' note:
  • Quote
  • Report post to moderator

Ellendra Nauriel wrote:Or, I just remembered, there are UV light sterilizing lamps available. I don't know which brand and style would work best for you, but it might be worth investigating.


Thanks Ellendra, I love the UV light idea! I am trying to sterilize the lab space, so this is a great solution. :-)

And yes, I already do have and use autoclaves and pressure cookers for sterilizing substrate growing media. :-)
 
Mike Kenzie
pollinator
Posts: 418
Location: wanderer
179
forest garden fungi foraging bike homestead
  • Mark post as helpful
  • send pies
    Number of slices to send:
    Optional 'thank-you' note:
  • Quote
  • Report post to moderator

Jamin Grey wrote:

Ellendra Nauriel wrote:Or, I just remembered, there are UV light sterilizing lamps available.



I actually got a brand new one of these, unopened, lying around here somewhere. Humana sent me one a few months ago due to the whole COVID panic.

These things are fairly cheap - about $25 online. I have zero idea how effective they are.

@MikeKenzie I would trade you my unused UV wand in exchange for two new species of edible/culinary (non-psychodelic) mushrooms I can grow on logs.

(I'm already growing portobella, winecap, lion's mane, and yellow oysters. I'd like two new ones)

I don't have any preference to the format you send it in (e.g. syringe, log, pegs, mason jar).


Thanks for the offer Jamin. You have a Purple Mooseage in your inbox. :-)
 
Mike Kenzie
pollinator
Posts: 418
Location: wanderer
179
forest garden fungi foraging bike homestead
  • Mark post as helpful
  • send pies
    Number of slices to send:
    Optional 'thank-you' note:
  • Quote
  • Report post to moderator

Ralph Sluder wrote:  You could make some strong vinegar with a small hand-full of raisins or whatever... takes less than a month to get some strong stuff.
Keep a 1/2 gallon going at all times and it will probably sterilize any surface very fast.


Yes! Great thinking! Change the pH dramatically! Do you have a favorite vinegar + raisins recipe that you like to use for cleaning?
Thanks in advance.
 
There's no place like 127.0.0.1. But I'll always remember this tiny ad:
GAMCOD 2025: 200 square feet; Zero degrees F or colder; calories cheap and easy
https://permies.com/wiki/270034/GAMCOD-square-feet-degrees-colder
reply
    Bookmark Topic Watch Topic
  • New Topic